Serial cloner clone in oligo12/27/2022 Compared to other factors influencing cloning success such as low competence of bacteria the benefit of higher quality oligonucleotides may not have a major impact, but for difficult clonings they may make the difference."ĭr. Therefore the more expensive Cloning Oligos have paid off. All these clonings were successful with a good rate of correct clones. "We have used the Cloning Oligos for several preparations of expression vectors. Kathrin Hölsch, TU München, Garching, Germany Translation of the original German statement Dr. The DNA sequences of two other colonies within the same plate were correct." But this oligo was very long with 58 bases in length - maybe that was the reason. "I am using your Cloning Oligos for direct cloning of short linker sequences, for PCR and subsequent cloning of the PCR products and site-directed mutagenesis.Overall I am very satisfied with the performance of your Cloning Oligos!We have had just one case this week that one cloning oligo has integrated a wrong base, what we have retrieved by sequencing. Peter Lorenz, Universitätsklinik Rostock, Germany It provides tools with an intuitive interface that assists you in DNA cloning, sequence analysis and visualization. Translation of the original German statement Dr. Serial Cloner for Linux is a Molecular Biology software. In this respect, I am very satisfied with the performance of these oligos….I could envisage to order the Cloning Oligos for similar applications in the future." Both clones whose plasmids we have sequenced were correct. For this, the original construct has been cut with HindIII/BamHI and then legated with the two complimentary flag-oligos, which generated the HindIII/BamHI overhangs. "The ordered Cloning Oligos were used to generate an expression construct with a flag-tag. Andreas Vogel, c-LEcta GmbH, Leipzig, Germany Translation of the original German statement Mr. Up to now they have been working perfectly. "We are using your Cloning Oligos for standard cloning. Angelika Ziegler, Julius Kühn-Institut, Bundesforschungsinstitut für Kulturpflanzen, Quedlinburg, Germany Overall, we are sure that the good quality of the oligos has furthered our research."ĭr. Subcloning to another vector was also successful, proving that the restriction sites within the oligos were functional. Sequencing revealed perfect 5' and 3' ends of the intended product. However, using the Cloning Oligos we generated amounts of full-length PCR product sufficient for cloning. Due to the very limited amounts of starting material we did not perform a comparison with conventional primers. "We have used the Cloning Oligos with incorporated restriction sites for RT-PCR amplification and subsequent cloning of a 9kb viral RNA. Teilen Sie uns Ihre Erfahrungen in unserem Feedback-Formular mit. Das sagen unsere Kunden über unsere Oligos
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